Fine Motion, the real life horse was intersex. This is reflected in the game as well TL;DR the horses have ear decorations. Left - girl. Right - boy. Fine Motion has decorations on both ears

Adric is nonbinary. Dev describes game as a non-binary western

The protagonist Rin is nonbinary. Working as a gig actor they get immersed in roles, will they stay true to themselves or start living another life?

The protagonist Starr is genderfluid and the game explores gender expectations

The game refers to the player neutrally as developer avoided using gendered terminology

Aldro does not specify gender on any NPC nor does it plan to

The protagonist can use she, him or they pronouns. The school mascots gender is never revealed in the first game The character Em is nonbinary and uses they/them pronouns

You can choose they/them pronouns

There are nonbinary, agender, and genderfluid pride flags in the shop. It's a bundle for 5 town points (basically free)

The game centers around subtle they/them representation by avoiding any form of binary characters - both in text and in visuals.